CAN_332

origin: Constraining enzyme conformational change by an antibody leads to hyperbolic inhibition.
author: Oyen, D., Srinivasan, V., Steyaert, J., Barlow, J.N.
patent: no
publication_number: not known
patent_application_number: not known
CDR1_seq: FTFNNYWMY(predict)
CDR1_length: 9
CDR2_seq: MINPGGIITK(predict)
CDR2_length: 10
CDR3_seq: DWATGLAK(predict)
CDR3_lentgh: 8
protein_seq: QLQESGGGLVQPGGSLRLSCAASGFTFNNYWMYWVRRAPGKGLEWVSMINPGGIITKYAESVKGRFTISRDNAKNTLYLQMNSLTSEDTAVYYCAKDWATGLAKKGQGTQVTVSS
DNA_seq: not known
PubMed_ID: 21238460
PDB_ID: 3K74
PDB_deposition_date: 2009-10-12
PDB_released_date: 2010-10-20
resolution: 1.95Å
source_organism: Lama glama
experiment_method: llama immunization,library construction,screen,express
antigen: DHFR(dihydrofolate reductase)
antigen_chain: not known
antigen_source: Escherichia coli
antigen_seq: MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR
epitope: not known
IEDB_ID: not known
epitope_reference: not known
function: a potent allosteric inhibitor of DHFR, giving rise to mixed hyperbolic inhibition kinetics
validationORprediction: Experiment Validation
bacteria_family: Escherichia coli
publication: J Mol Biol. 2011 Mar 18;407(1):138-48. doi: 10.1016/j.jmb.2011.01.017. Epub 2011 Jan 14.